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TT2020 Weizmann Institute of Science
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16th TT Meeting, 25-28 October, 2020
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Research The ISTT is involved in all levels of transgenic research in animals, from the animal technician to the senior scientist. This link will take you to our research page that includes relevant publications, CRISPR/Cas9 resources and news pertaining to transgenic technology. Training The ISTT sponsors meetings, workshops and training courses in transgenic technology. One of our most popular offerings is the Transgenic Technology (TT) meeting. To see the latest offerings, and to see how to apply for ISTT sponsorship of your training opportunity, please click here. Sponsor The ISTT is very grateful to our generous sponsors who provide us with support to continue our work. ISTT sponsors offer valuable goods and services necessary for transgenic technologies. The resources provided to the ISTT allow us to host the TT meetings, provide awards for our members, and assist with website support. Please visit our sponsors’ page.
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Single Copy Transgene Integration in ROSA26 Safe Harbor

The integration of single copy transgenes in a safe harbor permissive for gene expression will be satisfied by targeting transgenes to the mouse ROSA26 locus and the rat ROSA26 locus with Cas9 technology. Single copy transgene integration is essential to facilitate the analysis of endonuclease activity on transgenes. Conventional methods to produce transgenic mice and rats by pronuclear microinjection of linear DNA fragments in zygotes results in the multicopy transgene integrations in random sites (Brinster et al., 1985, Srivastava, 2014). Because resulting integration sites are randomly distributed over the genome no two founder animals identical integrated transgene alleles. Single copy transgene integration in a specific genomic target, ROSA26, will provide for facile, rapid analysis Cas9 chromosome break and repair events at defined sgRNA targets.

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Latest News

The ISTT responds to the ECJ Ruling that CRISPR/Cas9 should be regulated as GMO

Published Wednesday, August 15, 2018

The Board of Directors of the International Society for Transgenic Technology (ISTT) has the following comment on the ruling of the European Court of Justice (ECJ), which requires any that genome-edited organism shall be treated as GMO within the meaning of the European GMO Directive.

The ISTT, a scientific society representing the largest group of experts in the field of genome modification in animals, is surprised and disappointed that the court decided to focus on the method and not the product of genome editing and thus did not follow the recommendation of the court's Advocate General.

CRISPR Gene Editing of Human Embryos

Published Friday, August 4, 2017

In a collaborative effort of USA, South Korean and Chinese laboratories, the authors describe the correction of a mutation in the MYBPC3 gene associated with hypertrophic cardiomyopathy, through gene-editing, using CRISPR tools. (Nature article detailing results).

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