New Publications of Interest

CRISPR Technology

Simplified CRISPR tools for efficient genome editing and streamlined protocols for their delivery into mammalian cells and mouse zygotes (Quadros, Harms, Ohtsuka, Gurumurthy) [May 2017]
Generation of gene-edited rats by delivery of CRISPR/Cas9 protein and donor DNA into intact zygotes using electroporation (Menoret) [Nov. 2017]
Successful production of genome-edited rats by the rGONAD method (Ohtsuka) [April 2018]
An optimized electroporation approach for efficient CRISPR/Cas9 genome editing in murine zygotes (Troeder, Zevnik) [May 2018]
Electroporation of mice zygotes with dual guide RNA/Cas9 complexes for simple and efficient cloning-free genome editing (Teixeira, Markossian) [Jan. 2018; Correction in March 2018]
Efficient generation of targeted large insertions by microinjection into two-cell stage mouse embryos (Gu, Posfai, Rossant) [Nature Biotechnology 36(7):632-637, 2018]
CtIP fusion to Cas9 enhances transgene integration by homology-dependent repair (Menoret) [Mar. 2018]
i-GONAD: A robust method for in situ germline genome engineering using CRISPR nucleases. (Ohtsuka, Maiura, Gurumurthy) [Feb. 2018]
EASI-CRISPR: A robust method for one-step generation of mice carrying conditional and insertion alleles using long ssDNA donors and CRISPR ribonucleoproteins (Quadros, Harms, Ohtsuka, Mansour, Maiura, Gurumurthy) [May 2017]
CLICK: One-step Generation of Conditional Knock-Out Mice doi: 10.1186/s12864-018-4713-y [May 2018]
Redefining mouse transgenesis with CRISPR/Cas9 genome editing technology (Burgio) [Feb 2018]
Re-Evaluating One-step Generation of Mice Carrying Conditional Alleles by CRISPR-Cas9-Mediated Genome Editing Technology (Gurumurthy (1st author), many additional ISTT members as authors) [Sept 2018]




History of Cryobiology (Takeo, Nakagata) [April 2018]

Implantation, surgery, Husbandry

Reproductive Performance after Unilateral or Bilateral Oviduct Transfer of 2-Cell Embryos in Mice (Mahabir, Urban, Michel).  JAALAS 57(2):110-114 (2018).  [March, 2018]
Influence of polygamous versus monogamous mating on embryo production in four different strains of mice after superovulatory treatment (Michel, Mahabir). Theriogenology DOI:10.1016/j.theriogenology.2018.03.023 [March 2018]


Transparency in Animal Experimentation (Montoliu) [May, 2018]


(ISTT member authors)