TT2026

The 2nd Midwest CRISPR Animal Modeling Symposium (M-CAMS)

Date: March 13, 2026
Location: 
West Lafayette, Indiana

The 2nd Midwest CRISPR Animal Modeling Symposium (M-CAMS) will again be hosted by the Purdue Institute for Cancer Research-Transgenic and Genome Editing Facility (TGEF) and held in West Lafayette, Indiana on March 13, 2026

REGISTER HERE FOR THE MIDWEST CRISPR ANIMAL MODELING SYMPOSIUM MARCH 13, 2026
We had so much fun last time, we are doing it all again: a morning of talks by some of our most beloved ISTT members-Guru, Zach Freeman, Tristan Kempston, Masato Ohtsuka and special guest Mark Budde, founder of Plasmidsaurus, a lunchtime workshop on designing animal models and an afternoon dedicated to tech talk. The afternoon Protocol Parade and Technical Roundtable will again cover all our standard protocols (superovulation, microinjection, electroporation, embryo transfer, cryopreservation, IVF rederivation) and will also feature 2 focus sessions this year: One on zygote electroporation/AAV/GONAD and another on Sequencing/Bioinformatics for Genotyping. We will talk about what we do the same, what we do differently and why. What's not working, what's working really well and why. What's tried and true, what's new and what's been around but we've been afraid to try. Tricks we are delighted to spread tips we are happy to share and secrets we are willing to divulge. And then we will keep the conversation going and the good times rolling at our CRISPR Mixer!

March 12, 2026

Pre-Symposium Workshop : Ensembl – Genome Browser

Jorge Batista da Rocha (trainer)  -  Full day workshop

March 13, 2026

Morning Talks – Session 1 : Animal Model Design with CRISPR/cas9

Tristan Kempston  -  Introduction to Gene Editing with CRISPR-Cas9
Chanabasavaiah Gurumurthy  -  RE-CREATING Existing Mouse Models into Novel Animal Models
Masato Ohtsuka  -  Integrase/Recombinase-Mediated Targeted Transgenesis: PITT and SCRIPT
Stephane Pelletier  -  eSPLIT and iSWAP: Streamlined Strategies and Design Principles for Conditional Allele Engineering using Short Artificial Introns

Morning Talks – Session 2 : Sequencing for Genotyping and Validation

Mark Budde  -  Co-Founder/CEO of Plasmidsaurus, Special Guest – How Sequencing Enables a Step Change in Scientific Discovery
Zachary Freeman  -  Bridging Past and Future: Validating Classic and Next-Generation Engineered Models Using Long-Read Sequencing

Lunch and Learn : Animal Model Design Workshop

Tristan Kempston  -  Designing CRISPR-based Mutations in Rodent Models

Protocol Parade and Technical Roundtable : All Hands on Tech

Focus session 1  -  Sequencing/Bioinformatics for Genotyping and Validation
(Experts on Tap: Zach Freeman, Fabien Delerue, Plasmidsaurus, Hedyeh Rahimian)
Focus session 2  -  Zygote Electroporation/AAV/iGONAD
(Experts on Tap: Fabien Delerue, Nobuko Yamanaka, Yasuhiro Moriizumi (BEX), Michael Sperber (NepaGene), VectorBuilder, Masato Ohtsuko)
Other topics  -  Genome Browsers; Pipette pullers; anesthetic machines; ICSI; Needles; Rabbits; Rats; Gene Editing Software and more
Other Experts on Tap  -  Jorge Batista da Rocha (Ensembl); Adair Oesterle (Microtul); Kim Matheny (Kent Scientific); Jesse Toumeh (VectorBuilder) and YOU

Chat with Mark Budde  -  Mark has graciously agreed to have an inspirational, sit down chat with grad students and any other interested parties

Midwest CRISPR Mixer  -  Let the fun begin continue!

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