Long-Read Sequencing in Practice: Practical Strategies for Design, Multiplexing, and Analysis

ISTT Educational Initiatives series - online workshops

Long-Read Sequencing in Practice: Practical Strategies for Design, Multiplexing, and Analysis

CRISPR gene editing has been effectively employed to streamline the production of genetically modified animal models for biomedical research. However, a notable limitation is the potential for on-target mutations that may have detrimental consequences. These bystander mutations often go undetected with conventional genotyping methods, such as PCR, and standard sequencing techniques. Accurately identifying targeted animals is crucial for evaluating the efficiency of creating genetically engineered (GE) animal models. Long-read sequencing (LRS) has revolutionized this process by enabling comprehensive characterization without the need for PCR amplification. This advancement eliminates the need for multiple sets of junction PCRs, simplifying the verification of insertion accuracy and aiding in the screening for random integrations.

Presenter: Monica Sentmanat

23 February 2026 at 13:00 UTC | 10AM EDT | 4PM Paris | 7:30PM New Delhi

24 February 2026 at 20:00 UTC | 5PM EDT | 11PM Paris | 6AM Seoul | 7AM Sydney

Register HERE

• Free for ISTT members - with code (see registration page for link)
• $50 for non-members - payment accepted on the registration page
• $30 for IMGS members - with code (see registration page for link)